Coding

Part:BBa_I746917:Design

Designed by: Stefan Milde   Group: iGEM07_Cambridge   (2008-09-30)


P7 GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644


Design Notes

PCR was carried out from plasmid DNA followed by standard assembly into several constructs.


Source

P7 GFP was created and described by: Fisher et al(2008): "Laboratory evolution of fast-folding green fluorescent protein using secretory pathway quality control", PLoS ONE 3(6)

It was biobricked by PCR and standard assembly from DNA kindly provided by Dr Adam C Fisher, Cornell University, New York.

References